Optical microscopy has limited application in 3D bio-tissue modeling due to the intensive data manipulation required. For example, to examine a sample with high resolution using optical microscopy it must be physically sectioned onto very thin slides. The division into these slides is labor intensive and the resulting images of the target organ would be a series of thousands of 2D images that must be digitally stacked into 3D columns. Due to excessive computation and a memory-intensive process it is a significant challenge to train computers to identify individual cells by their visual characteristics, even with the aid of complex staining. However, to date, differentiating tissue down to the level of the individual cell may still be only possible using optical microscopy.