PCR is a modern technique used in molecular biology labs that uses thermocycling and enzymes to quickly and reliably copy DNA. With the development and breakthrough of science and technology, PCR technology has been widely used in many fields, such as microbial detection, veterinary medicine, aquaculture and so on. Its application field is extending continuously as this technique has strong sensitivity, accuracy , and specificity and it can be detected quickly. On the basis of conventional PCR technologies, many new PCR technologies such as extreme PCR, photonic PCR, o- amplification at lower denaturation temperature PCR, nanoparticle PCR and so on have been developed. PCR is used in molecular biology to make many copies of (amplify) small sections of DNA or a gene. Using PCR it is possible to generate thousands to millions of copies of a particular section of DNA from a very small amount of DNA.
PCR is a common tool used widely in medical and biological research labs in the early stages of processing DNA for sequencing, for detection of the presence or absence of a gene to help identify pathogens during infection, and when generating forensic DNA profiles from tiny samples of DNA. In the context of modern accurate diagnosis, how to improve the technology, ensure high sensitivity and specificity while improving and the ease of use of the repeatability of PCR detection, will become the new direction of the future development of PCT technology; PCR technology and sequencing technology integrated automation will likely become the new requirements of clinical molecular diagnosis and public health emergencies nucleic acid detection.
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