Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through a sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelengths. In the broadest terms, it can be said the spectrometer is an instrument that can be used for measuring the variation of a physical characteristic over a provided spectrum. In the case of the mass spectrometer, this can be a mass-to-charge ratio spectrum, the varied range of nuclear resonant frequencies in an NMR spectrometer, or the change in the absorption and emission of light with a wavelength in an optical spectrometer. The most commonly used type of spectrometers that are used for research is optical spectrometers.
The main object of an optical spectrometer is to measure the reflection, absorption, and scattering of electromagnetic radiation with the emission of electromagnetic radiation from a sample or with the sample itself. They are mainly concerned with the electromagnetic radiation which falls within the optical region of the electromagnetic spectrum which is light spanning the visible, ultraviolet, and infrared wavelength regions of the spectrum. The emission of light or the interaction must be measured as a function of wavelength in order to gain maximum information and the common feature of all-optical spectrometers is thus a mechanism for wavelength selection. In cases where accurate wavelength selection is not important or in low-cost spectrometers, utilization of optical filters is done for isolating the wavelength region of interest. But a dispersive element that separates light into its constituent wavelengths is required for the generation of spectra and accurate wavelength selection. This dispersive element is a diffraction grating in which destructive and constructive interferences are used for spatially separating polychromatic light that is incident on the grating in the modern spectrometers. Monochromator which is a device that is used for selecting a particular wavelength of light from a polychromatic light source, diffraction gratings are a key component. The diffraction grating is rotated in a monochromator for changing the wavelength which aligns with and passes via the exit silt. In all spectrophotometers, excitation monochromators are found for selecting the desired wavelength for reaching the sample from a white light source and by measuring the change in signal as a function of excitation wavelength and by scanning the monochromator the spectra are measured. Dutco Tennant LLC and BCL bring to the Middle East and UAE a wide range of spectrometers from some of the leading brands of the world.
Here at Dutco Bcl, we have numerous Spectrometers for all your needs.