Before commencing the sample analysis procedure with the help of sophisticated scientific instruments and equipment, they are needed to be suitably treated and prepared. The analysis of samples in their original form may not deliver the most optimised end results.

The use of a high-efficiency sample preparation system is very much in demand in laboratories. In this blog, we discuss the basics of sample preparation to better understand the functions of a sample preparation system.

Keep reading till the end to find out more about sample preparation.

Overview

Sample preparation is a preliminary step in the overall sample analysis process. It is an important stage that aids in the prevention of contamination during analysis and allows improved accuracy while minimising the risk of distortion in final outcomes.

A majority of sample preparation tasks begin with extraction. In extraction, the isolation of a representative piece of material from a larger source is carried out. In a broader perspective, the sample preparation is done to enhance the suitability or quality of the sample and in turn also improve the subsequent analytical outcomes.

What is the point of  conducting Sample Preparation?

The answer to this can be broken down in the following points -

  • It helps in eliminating interferences from a given sample with the purpose of increasing selectivity during separation and identification.
  • It enables increasing sensitivity and concentration of the analyte.
  • It delivers conversion of analytes to a more desired form of detection, determination or separation.
  • Helps smaller sample sizes for trace analysis.
  • Allows application of reproducible techniques that can be utilised for all variations of a sample matrix.
  • Incorporate online techniques and automation to minimise manual procedures.
  • Aims to achieve higher specificity and selectivity.
  • Minimises the use of organic solvents.
  •  Brings down the production of wastes.

Techniques used for Sample Preparation

Based on the characteristics of the sample being analysed, there are varying techniques available and used. Some of the latest techniques in sample preparation are -

Solid-Phase Extraction

Solid-Phase Extraction utilises a solid absorbent for isolating compounds in a sample as per to their unique physical and chemical properties. It is one of the most extensively used methods for “cleaning up” samples for analysis and is used across a range of scientific fields. 

There are variations in this method such as Solid Phase Analytical Derivatization (SPAD) which are used in more specialised applications and deliver several perks, including minimised organic solvent consumption, increased efficiency,and enhanced compatibility with chromatographic systems. 

Gravimetric Sample Preparation

This technique involves sample preparation gravimetrically. It not only boosts accuracy and precision, but also gives access to huge time saving benefits for laboratories. 

This is an automated method that sees sample concentrations expressed in mg/g, with solvent added based on the weight of the solid sample.

If you are interested to learn more about sample preparation or looking for quality sample preparation systems then get in touch with the expert suppliers now.